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Caspases 3 and 9 are activated by eGFP/NS1 expression. HepG2 cells were transfected with either eGFP/NS1 or eGFP alone. The caspase activity markers DEVD-red and LEHD-red were used to assay for caspase 3 and caspase 9 activity, respectively. Cells with active caspases fluoresce red in this assay. Cells expressing eGFP/NS1 demonstrated active caspases 3 and 9, while activity of these caspases was not observed in cells expressing eGFP alone.
