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. 2006 Apr;80(8):4017–4025. doi: 10.1128/JVI.80.8.4017-4025.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 3. — Mutually exclusive binding of BMS-488043 and sCD4 to gp120_JRFL. (A) Effect of the order of BMS-488043 and [¹²⁵I]sCD4 addition. Symbols: +, BMS-488043 added to gp120 along with [¹²⁵I]sCD4; ○, [¹²⁵I]sCD4 added to gp120 before BMS-488043. gp120-[¹²⁵I]sCD4 was bound to lectin-resin and counted in a gamma counter. (B) Blocking of [³H]BMS-488043 binding to gp120 by sCD4. gp120 was treated with a mixture of [³H]BMS-488043 and sCD4 and then subjected to gel filtration, and gp120-bound [³H] activity was determined in a scintillation counter.