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VP4, the spike rotavirus protein, localizes within filaments and dots in infected and transfected Cos-7 cells. Cos-7 cells were infected with the rotavirus at 1 PFU/cell for 4 h (A) or transiently transfected with plasmids encoding VP4 (B) or EGFP-VP4 (C) for 24 h. Cells were then processed for VP4 staining (A and B). Images gallery displays projections of all 0.5-μm xy focal sections taken throughout the height of the cells. Scale bars = 10 μm.
