FIG. 6.

VP4 remodels actin microfilaments into actin bodies. EGFP-VP4 expressing Cos-7 cells were mounted in a POC chamber and cells were perfused with 37°C 50 mM HEPES and OPTI-MEM medium. Images were acquired with a confocal microscope from time zero (To) to 15 min after perfusion (T+15min) every 3 min. Image gallery displays projections of all 0.3-μm xy focal sections taken throughout the height of a zoomed region of a EGFP-VP4-expressing cell. Scale bar = 10 μm.