FIG. 7.

VP4 localizes at the brush border and perturbs actin bundles organization. EGFP-VP4 expressing Caco-2 cells were labeled 72 h after nucleofection with Alexa 547-phalloidin. Image gallery displays an apical plane these cells (A) or xz section (B). Fifteen-day-old Caco-2 cells were infected with rotavirus for 6 h (C through G) or for 18 h (H and I). A 1-h cytochalasin D (cytoD) (10 μM) treatment was performed after at 5 h p.i. (F and G). Cells were then processed for Alexa 547-phalloidin labeling and VP4 immunostaining using MAb 7.7. Images gallery displays apical planes (A, C, F, and H), basal planes (D and G) or xz sections (B, E, and I). Scale bar = 5 μm.