FIG. 8.

Actin is coimmunoprecipitated with VP4 from 18-h-infected Caco-2 cells. Differentiated Caco-2 cells were infected with rotavirus at 10 PFU/cell. Lysates from mock-infected or 18-h-infected Caco-2 cells were subjected to immunoprecipitation with anti-VP4 antibodies and then to Western blotting using anti-VP4 and anti-actin. Lane a, mock-infected Caco-2 cells lysates immunoprecipitated with anti-VP4 antibodies; lanes b and c, infected Caco-2 cells lysates immunoprecipitated with nonrelevant antibody and anti-VP4 antibodies, respectively. Note that immunoglobulins were not detected because anti-VP4 was covalently linked to protein A-Dynabeads.