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. 2006 Apr;80(8):3752–3764. doi: 10.1128/JVI.80.8.3752-3764.2006

FIG. 5.

FIG. 5.

Electron micrographs of HEL cells harvested 24 h after infection. Replicate cultures of HEL cells were exposed to 10 PFU of wild-type HSV-1(F) (F), R7041(ΔUS3) (A, B, and C), or R2640 [HSV-1(F)US3.5] (D and E); harvested 24 h after infection; and processed as described in Materials and Methods. The arrows marked a in panel A indicate the presence of enveloped particles in intranuclear vesicles. The arrows marked b in panel A indicate separation of inner and outer nuclear membranes. The arrows marked c in panels B, D, and E indicate multiple small sites where inner nuclear membrane became extended into the nucleus and formed vesicles anchored to the nuclear envelope. The arrow marked d in panel D indicates membrane reduplication. The arrow marked e in panel E indicates a capsid that appeared to undergo envelopment at a membrane site. The arrows marked f in panel F indicate “spikes,” or protrusions, on the nuclear membrane of the infected HEL fibroblasts. Magnifications: panel A, ×20,000; panels B, C, D, and E, ×40,000; panel F, ×15,000.