FIG. 5.

Distribution of nsP3/GFP and nsP2 in the cells infected with SIN/389. BHK-21 cells were infected at an MOI of 20 PFU/cell. At 8 h postinfection, cells were harvested, homogenized, and further fractionated as described in Materials and Methods. Aliquots of the fractions that were used for gel electrophoresis are indicated. After transfer, the nitrocellulose membranes were processed by rabbit anti-nsP2 or anti-nsP3 antibodies and horseradish peroxidase-conjugated secondary donkey anti-rabbit immunoglobulin G antibodies. The preparation of protein fractions is described in Materials and Methods. Sucr., sucrose.