FIG. 5.
Effects of mutations in nsP2. The effects of three mutations in nsP2 were evaluated using either a replicon expressing the luciferase gene (white bars) or mutated infectious cDNA clone (black bars). Data are presented as log10 of arbitrary emitted light units or PFU/ml for LUC activity and virus titer after one passage in cell culture. Amino acid mutations are indicated in the form of XnY, where X is the wild-type amino acid, n is the amino acid position in the nsP2 protein, and Y is the mutated amino acid. The wild-type nsP2 sequence (wt) is indicated. The results presented are the means of two individual experiments.