FIG. 5.
Infectivity of lef-4 mutant viruses. S. frugiperda cells were transfected with BacP+ (A and E), BacP+/LEF-4CmR (B and F), or BacP+/LEF-4CmR in the presence of plasmids encoding wild-type LEF-4 (C and G) or a mutant version of LEF-4 that lacks guanyltransferase activity (D and H). Cells were harvested at 12 or 48 h p.i. and processed for immunofluorescence using a mouse monoclonal antibody raised against IE1. Images were collected using a Zeiss ApoTome/Axioplan 2 microscope. Images shown represent merged images of phase contrast and green fluorescence to visualize percentages of cells expressing IE1.