TABLE 1.
PCR amplification and sequencing primers
ORF | Amplification primers
|
Sequencing primer(s)
|
||
---|---|---|---|---|
Name | Sequence (5′-3′) | Name | Sequence (5′-3′) | |
8 | 8f | AATTATTGGTGGGCAGAACC | 8s | CCATCACCACGCTATTCTC |
8r | TTCTCCAGCACAGCAACG | |||
11 | 11f | CTGTAACGATGCTAACGCTACG | 11sf1 | TAGCCCAAACGACGACAAAG |
11r | TTCAGAAACCTGTCCAGGTCC | |||
15 | 15f | CAAAGCGCAAAGCAAAATGC | 15sf1 | ATCCAACACACGCAACATC |
15r | GGGCCTTGTAGATCAGCTCTCC | |||
30 | 30f1 | GCGCTACTTCTGAAAACG | 30-1s | TACCCAAGCATTATCCAG |
30r1 | CCACAAACTTGATAAACACG | 30-1sr1 | GATAACCCTGACGGAGTAAG | |
30f2 | AAACCACAAGCCTTACTCC | 30-2s | GAGAAGACCTTTCAGCGAC | |
30r2 | GCGCAACTATCACATACG | 30-2sr1 | CTCAGCAGTCATAACGAAC | |
31 | 31f | GGACTTACCGTCGAACAGACC | 31sr1 | GGAGACAGGTTGAGAGAAG |
31r | ACCCAGTACGTAGCCATGAAGC | 31sr | CAGCCTCGCAAGCGCGGGTGAGGT | |
32 | 32f | TGATTACTACTACCCCCCTCC | 32sf1 | TATTTCTGACACCCACCAC |
32r | AGCCAGATTCACGTCATCC | |||
37 | 37f | ACATACTCCGCCATCTCC | 37s | CCATCTATAACGCCAGCC |
37r | ACAGCTACATGACTCGTGC | |||
40 | 40f | ATCACAAACGCATCCTCG | 40s | ACTCGCCGAATCCCAGATG |
40r | CACAGCTCACCCAAAATACC | |||
42 | 42f1 | ATCCCAGCGCAACTCTTAC | 42fs1 | GGCAAGTATAACCTGAACGG |
42r1 | GTCCGTAGAGCTGAGTAGAG | 42rs1 | TGAAACAGCGAACACGGGTC | |
45 | 45f | TGGCACGAAAGCACTACG | 45sr1 | TGATCTATCAGAGCCAAC |
45r | AAACCTGCACGAATGTTGG | |||
50 | 50f | ACTCGACTCAGACTACATTGG | 50sr1 | GCTCACTAAATAGCCAAAC |
50r | ATAGCAGAATGGGGATAGC | |||
52 | 52f | CCAACAGCTTTCACATCACC | 52s | GCTACTTCGTTCACTTCC |
52r | TTCAAATTCCTCGCTCACC | |||
57 | 57f1 | GGTGCTGAACGTAAACCTG | 57fs1 | CGTAAACCTGAGCGAG |
57r1 | CTCAGGGCTGCTAATATATGC | 57rs1 | GTAGAAACCTTGACGACACGG | |
67 | 67f | CAAAACTCCAACACCACG | 67s | GAAGAAGCATTCGCCAAG |
67r | GAGAAGAGGTTCTACAAGCAGC | |||
68 | 68f | AGCATTGCCAAACAGTTCC | 68s1 | GAAGATAGAATGGGTGTGAG |
68r | CAAGAAACCACTGCTCAACC | 68s3 | ACCGTTGAGCATAATCATCC | |
68r2 | GTCCCCTACCTTTTAACG | |||
68r3 | CGGCGGCCGTGGACCGA | |||
73 | 73f | GAACATATGAGCATGTACC | 73s | ACGGACAACGCTTACCCAC |
73r | GACCAGTAGAACATCTTCC |