FIG. 4.
Mutant agnoproteins are expressed during the infection cycle. (A) Analysis of WT and mutant agnoproteins during the early phases of infection cycle. The Mad-1 WT genome or its phosphorylation mutants of agnoprotein (Mut1, Mut2, and Mut3) were separately transfected into SVG-A cells (8 μg of DNA/2 × 106 cells/75-cm2 flask). Whole-cell lysates were prepared at the indicated time points and analyzed by Western blotting (40 μg/lane) with an anti-agnoprotein antibody. A hatched arrowhead in each panel points to a nonspecific band, which was detected by using an anti-agnoprotein antibody. (B) In parallel, whole-cell lysates were also analyzed for agnoprotein expression for the later days of infection cycle by Western blotting as described for panel A.