FIG. 1.

Identification of the region of CPSF30 that binds to the 186-amino-acid region of the NS1A protein. (A) GST fusions containing the indicated regions of CPSF30 were mixed with ³⁵S-labeled wt NS1A protein, followed by affinity chromatography on glutathione-Sepharose. (B) GST-F2F3 or GST was mixed with ³⁵S-labeled wt or M186 mutant NS1A protein as indicated, followed by affinity chromatography on glutathione-Sepharose (lanes 3 to 5). Lanes 1 and 2 show the input wt and M186 NS1A proteins. RBO, RNA-binding domain.