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. 2006 Apr;80(8):3985–3993. doi: 10.1128/JVI.80.8.3985-3993.2006

FIG. 4.

FIG. 4.

Total DC cell numbers in the draining popliteal lymph nodes and spleens of HSV-1-infected animals. PLN and splenocyte cells were harvested from mice on day 4 post-HSV-1 infection and surface stained for expression of CD11c, CD8a, and B220. Representative histograms depicting the typical frequency of CD11c+ cells observed on day 4 post-HSV-1 infection in the PLNs and spleen of PBS-treated, DT-treated, and nontreated DTR-Tg mice are depicted in panels A and B, respectively. (C and D) Total cell numbers were calculated by multiplying the total percentage of CD11c+ cells by the number of total cells per PLN or spleen. pDC numbers were calculated by multiplying the total percentage of CD11c+ B220+ cells by the number of total cells per PLN. cDC numbers were calculated by multiplying the total percentage of CD11c+ CD8a+ cells by the total number of cells per PLN. Each bar represents the mean value obtained from a minimum of two experiments using a minimum of three mice per treatment. The error bars represent the standard deviation from the mean.