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. 2006 Apr;80(8):3985–3993. doi: 10.1128/JVI.80.8.3985-3993.2006

FIG. 7.

FIG. 7.

DC depletion impairs CD4+ T-cell activation. PLN and splenocyte cells were harvested from day 4-infected mice and surface stained for expression of CD4 and CD25. Representative histograms depicting the typically observed frequencies of activated CD4 T cells in the PLNs and spleen of PBS-treated, DT-treated, and nontreated DTR-Tg mice on day 4 postinfection are shown in panels A and B, respectively. Numbers in the upper right quadrant represent the percentage of activated T helper cells gated in the rectangle. (C and D) Total cell numbers were calculated by multiplying the percentage of double-positive cells in the rectangular gate by the number of total cells per PLN or spleen. Each bar represents the mean value obtained from a minimum of two experiments using a minimum of three mice per treatment. The error bars represent the standard deviation from the mean. Statistical analysis using the Wilcoxon sum of ranks test showed a significant difference on day 4 p.i. PBS plus HSV-1 versus DT plus HSV-1 (P ≤ 0.05) and HSV-1 versus DT plus HSV-1 (P ≤ 0.05) DTR-Tg mice are shown.