FIG. 3.

Deletion of CCPs from bovine CD46 results in loss of BVDV binding and no increase of susceptibility to BVDV. SK6T cell lines that express CD46 with deletions (Δ) of one ore more CCPs were established. (a) Cells were grown for 24 h in the absence and presence of 5 μg/μl Dox, and crude cell lysates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis using anti-CD46 serum. Observed differences in molecular weight are due to deletion of CCPs containing different numbers of potential N-linked glycosylation sites. (b and c) Binding of ³H-labeled BVDV (b) and susceptibility to BVDV (c) were assayed in the absence and presence of Dox. The columns represent mean values of duplicate experiments; error bars indicate maximum and minimum values.