FIG. 5.

The presence of the porcine hexapeptide A₈₃LPTFS₈₈ within CCP1 of bovine CD46 results in a loss of receptor function. (a) Sequence alignment of CCP1 of both bovine and porcine origin. Shaded amino acids were replaced by the corresponding porcine sequences throughout this study. The N-terminal and C-terminal halves of CCP1 are indicated. (b) SK6T cell lines were constructed that express chimeric CD46 molecules in which parts of CCP1 from bovine CD46 were replaced by the analogous sequences from porcine CD46. The susceptibility to BVDV was assayed in the absence and presence of Dox. The columns represent mean values of duplicate experiments; error bars indicate maximum and minimum values.