FIG. 8.

The distance between the virus binding domain and the plasma membrane influences the receptor function. SK6T cell lines were constructed that express chimeric CD46 molecules containing an increasing number of additional CCPs from C4bp between the STP region and CCP4. (a) Cells were grown for 24 h in the presence of 5 μg/μl Dox, and crude cell lysates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis using anti-CD46 MAb BVD/CA 26. (b) Susceptibility to BVDV was assayed in the absence and presence of Dox. The columns represent mean values of duplicate experiments; error bars indicate maximum and minimum values.