TABLE 1.
Primers used in this studya
| Primer | Nucleotide sequence |
|---|---|
| GP5-For | 5′ GCCGGAATTCGGAGCCGCCGCCACCATGTTGGGGAGATGCTTGAC 3′ |
| GP5-Rev | 5′ CCCCGAATTCCTAAAGACGACCCCATTGTTC 3′ |
| M-For | 5′ ATATATGGATCCGCCACCATGGGGTCGTCTTTAGACGAC 3′ |
| M-Rev | 5′ ATATATGCATGCTTATTTGGCATATTTGACAAGG 3′ |
| GP5-N34A-For | 5′ GCCAACAGCGCCAGCAGCTCTC 3′ |
| GP5-N44A-For | 5′ GTTGATTTACGCCTTGACGCTATG 3′ |
| GP5-N51A-For | 5′ GTGAGCTGGCTGGCACAGATTG 3′ |
| GP5-N34/44A-For | 5′ GCCAACAGCGCCAGCAGCTCTCATCTTCAGTTGATTTACGCCTTGACGCTATG 3′ |
| GP5-N44/51A-For | 5′ GTTGATTTACGCCTTGACGCTATGTGAGCTGGCTGGCACAGATTG 3′ |
| GP5-N34/51A-For | 5′ GCCAACAGCGCCAGCAGCTCTCATCTTCAGTTGATTTACAACTTGACGCTATGTGAG CTGGCTGGCACAGATTG 3′ |
| GP5-N34/44/51A-For | 5′ GCCAACAGCGCCAGCAGCTCTCATCTTCAGTTGATTTACGCCTTGACGCTATGTGAG CTGGCTGGCACAGATTG 3′ |
| PRRSV-13177-For | 5′ CTACCAACATCAGGTCGATGGCGG 3′ |
| PRRSV-14473-Rev | 5′ GTCGGCCGCGACTTACCTTTAGAG 3′ |
a
Underlining indicates mutations. Restriction enzyme sites incorporated in the primers are shown in bold; the initiation codons for the GP5 and M proteins are shown in bold italics.