FIG. 7.

Q548R mutant IE86 protein is unable to arrest cell cycle progression in infected HFF cells. (A) Serum-starved, nonconfluent HFF cells were synchronized in G₀ for 48 h. Cells were infected for 6 h in low-serum medium with Towne, Q548A, or Q548R RV at an MOI of approximately 0.9. Cells were then grown in high-serum medium to promote cell cycle progression for 42 h. Cells were permeabilized in 70% methanol, stained with anti-IE86 antibody, counterstained with DAPI, and subjected to FACS analysis. IE86-positive infected cells were sorted and counted based on DNA content. (B) Cell cycle profiles from the above experiment were analyzed using ModFit software to determine the percentage of infected cells in each phase of the cell cycle for each sample.