FIG. 8.

Q548R mutant IE86 protein is able to upregulate E2F-responsive genes in infected HFF cells. Confluent, contact-inhibited HFF cells were infected, in triplicate, with equivalent CPE input of Towne, Q548A, or Q548R RV, equivalent to an MOI of approximately 0.3. Cytoplasmic RNA was harvested at 48 hpi and separated on an agarose-formaldehyde-MOPS gel. RNA was transferred to a Nytran membrane, and Northern blot analysis was performed using ³²P-labeled DNA probes for the E2F-responsive genes Cdk2, MCM3, RR1, and TS and the cellular gene actin.