FIG. 1.

Daxx is degraded early during HCMV infection. (A) HFs were uninfected (−) or infected (+) with HCMV at an MOI of 3. Lysates were harvested at the indicated hours postinfection (hpi) and analyzed by Western blotting. Tubulin (Tub) was analyzed as an internal loading control. (B) HFs were either mock infected (M) or infected with HSV-1 or HCMV at an MOI of 3. Lysates harvested at 4 hpi were analyzed by Western blotting. (C) HFs were treated as in panel A. pp28 is an HCMV protein that serves as a marker for the late stages of viral infection.