FIG. 2.

Daxx is degraded by a tegument protein in a proteasome-dependent manner. (A) HFs infected with HCMV at an MOI of 3 were treated with DMSO (D), lactacystin (L), or E64. Lysates were harvested 6 h postinfection (6 hpi) and analyzed by Western blotting. Tub, tubulin. (B) HFs were mock infected, infected with HCMV (V), or infected with HCMV particles exposed to UV light (UV-V) at an MOI of 3. Lysates were harvested at 6 hpi and analyzed by Western blotting. (C) Untreated HFs or HFs preincubated with cycloheximide (C) were mock infected or infected with HCMV at an MOI of 3. Lysates were harvested at 6 hpi and analyzed by Western blotting. (D) HFs were mock infected, infected with HCMV, or infected with HCMV treated with heparin (hep) at an MOI of 0.1. Lystates were harvested at 6 hpi and analyzed by Western blotting. (E) HFs were mock infected or infected with purified HCMV particles (PV) at an MOI of 3. Lysates were harvested at 6 hpi and analyzed by Western blotting.