FIG. 6.

Daxx silences the HCMV MIEP. (A) Mock-infected HFs (M) or HFs infected with HCMV at the indicated MOIs were treated with DMSO (D) or lactacystin (L). Lysates were harvested 6 h postinfection (hpi) and analyzed by Western blotting. Tub, tubulin. (B) HFs transfected with siRNA directed at either Daxx or Skp-1 and subsequently infected with HCMV were treated with either DMSO or lactacystin. Lysates were harvested 6 hpi and analyzed by Western blotting. Lysates from HCMV-infected (MOI of 0.1) and mock-infected cells not treated with siRNA were also analyzed.