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. 2006 Apr 20;116(5):1337–1345. doi: 10.1172/JCI27400

Figure 5. CD2AP forms an endogenous complex with Fyn and Synpo.

Figure 5

(A) Endogenous CD2AP interacts with endogenous Fyn in cells (upper panel) and rat glomeruli (lower panel). Lysates were prepared from HEK 293 cells or isolated rat glomeruli, respectively (input lysate, 25 μl out of 800 ml; lane 1). Immunoprecipitates were prepared with a control antibody (rabbit serum, lane 2) or with polyclonal antisera to Fyn (lane 3). Immunoblotting was performed with a polyclonal antibody to CD2AP. (B) Endogenous CD2AP interacts with endogenous Synpo in podocytes. Lysates were prepared from differentiated podocytes. Immunoprecipitates were prepared with a control antibody and anti-Synpo (upper panel) or anti-CD2AP (lower panel) antibodies and immunoblotted with anti-Synpo or anti-CD2AP.