Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reverse transcriptase—polymerase chain reaction (RTPCR) assay of the stop codon region of the outer membrane protein A (ompA) gene of Rickettsia felis using primer set 3 (nucleotides 1836—2180). Molecular sizes were determined by a 100-basepair DNA ladder. Lane1, RT/PCR product of R. felis RNA; lane 2, genomic PCR; lane 3, water; lane 4, PCR of R. felis RNA without RT (control for DNA contamination). Kbp = kilobasepairs.
