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Substitution of Ba2+ for Ca2+ eliminated most of the enhancement of Iin elicited by IF in OSNs. (A) Under the single-voltage-pulse protocol, the peak of Iin increased with repetitive recordings in APS (solid circle). When Ca2+ in the bath was replaced by Ba2+, majority of Iin elicited by IF disappeared and the increase of Iin was decreased (open circle) (n = 5). (B) Similar results were observed with the dual-step protocol with same cells as A after a 5-min rest (n = 5).
