Figure 5.

Ca²⁺ chelators decreased the enhancement of I_in elicited by IF in OSNs. (A) The IF-elicited response as measured with the single-pulse protocol in APS, Ba²⁺-APS, and BAPTA-AM–Ba²⁺-APS. I_in increased with repetitive recordings in APS (solid circle). In Ba²⁺-APS, a large portion of the potentiation was eliminated. Inclusion of a membrane-permeable Ca²⁺ chelator, BAPTA-AM, further reduced the potentiation response (n = 3). (B) Using whole-cell recording with K⁺-BAPTA, adenosine triphosphatase, and guanosine triphosphate after the membrane was ruptured, a single OSN was stimulated with IF first by single-pulse protocol (solid circle) and then by dual-pulse protocol (open circle). Similar results were observed in four other cells.