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Bath solutions (in millimolars)
| NaCl | HEPES | KCl | CaCl2 | BaCl2 | Glucose | Na-gluconate | BAPTA-AM | |
|---|---|---|---|---|---|---|---|---|
| APS | 112 | 3 | 2 | 8 | 5 | |||
| Low Cl APS | 20 | 3 | 2 | 8 | 5 | 92 | ||
| Ba2+-APS | 112 | 3 | 2 | 8 | 5 | |||
| BAPTA-AM-APS | 112 | 3 | 2 | 8 | 5 | 0.05 |
All solutions were at pH 7.2. NA-APS was APS + 300 μM niflumic acid.
