Skip to main content
. 2006 Feb 24;90(10):3570–3581. doi: 10.1529/biophysj.105.078295

FIGURE 2.

FIGURE 2

Effect of depolarizing conditioning potentials (CP) on wild-type CLH-3a and CLH-3b. (A) Representative current traces from HEK293 cells cotransfected with GFP and mutant channel cDNAs. Cells were held at conditioning voltages of −20 mV to 60 mV for 3 s and then voltage clamped at −120 mV to activate heterologously expressed channels. A schematic diagram of the wild-type CLH-3a and CLH-3b channels is shown above the current traces. (B) Effect of conditioning potential (CP) on hyperpolarization-induced current activation. Peak current amplitude was measured between 170 ms and 270 ms after stepping to −120 mV. Current values were normalized to that measured following a conditioning pulse of −20 mV (i.e., I−20 mV). Values are means ± SE (n = 6–7). (C) Effect of CP on current inactivation. Mean normalized pseudo-steady-state current (ISS) was measured over the last 20 ms of the −120 mV test pulse and normalized to peak current (Ipeak) amplitude. Values are means ± SE (n = 6–7). Predepolarization induces current potentiation and inactivation only in CLH-3a.