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. 2006 Feb 24;90(10):3672–3685. doi: 10.1529/biophysj.105.067793

FIGURE 1.

FIGURE 1

Schematic of RNA-tethered fluorescent microspheres in a shallow-penetrating evanescent field (shaded) generated by total-internal reflection fluorescence (TIRF) illumination. The fluorescent microspheres are streptavidin coated and bind tightly to the 3′-biotinylated RNA tether. The 5′ terminus of the RNA is fluorescein labeled and adheres to the glass substrate via goat anti-fluorescein IgG. Upon addition of magnesium ions and subsequent compaction of the RNA tether, the Brownian motion of the microsphere becomes attenuated.