Figure 4.

Tip60 knockdown does not affect p53 activation by UV-C. (A) U2OS cells were transfected by the indicated siRNAs and UV-irradiated (10 J/m²) 48 h later, where indicated. After 16 h, total cell extracts were prepared and analysed by Western blot for the presence of p53 (upper panel). After stripping, the membrane was blotted using an antibody recognising HDAC1/2 (lower panel). (B) Same as in (A), except that total cell extracts were analysed by Western blot for the presence of S15-, S37- and S392-phosphorylated p53. The stars indicate nonspecific bands.