Figure 7.

p400 knockdown induces a cell cycle arrest. (A) U2OS cells were transfected with the indicated siRNAs by electroporation. After 48 h, total cell extracts were prepared and analysed for the presence of p21 or HDAC (as a loading control) by Western blot. (B) Same as in (A), except that cell extracts were analysed for the presence of Rb by Western blot and for equal loading by ponceau red staining. (C) U2OS cells were transfected with the indicated siRNAs by electroporation. After 48 h later, the cell cycle distribution of transfected cells was analysed by flow cytometry.