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. 2006 Apr 6;25(8):1764–1774. doi: 10.1038/sj.emboj.7601045

Figure 2.

Figure 2

ATM and ATR inhibit DSB accumulation in the presence of damaged replication forks. DSBs detected by TUNEL labeling of postreplicative chromatin isolated after 120-min incubation in control extracts (A, Control), in extracts supplemented at 0 min with 4 ng/μl of Geminin (B, GEM), 500 μM Roscovitine (C, High Rosc) or 5 μM Roscovitine (D, Low Rosc). Extracts were supplemented at 60 min from nuclei addition with buffer (Buffer), with 55 μM CPT or with 300 μM MMC. Extracts were mock depleted (gray bars), supplemented with 5 mM caffeine (black bars), ATM and ATR depleted (white bars) or untreated (striped bars).