Figure 6.

Brm is not involved in Tat activation of a nonintegrated LTR template. (A) HeLa cells were transfected twice with siRNA against Brm, P300 or control siRNA. The levels of endogenous Brm, P300 and actin were determined by Western blot analysis. (B) At 24 h after siRNA treatment, cells were transfected with an expression vector for Tat-Flag and the LTR-Luc reporter gene. The luciferase activity was determined 24 h after Tat transfection, and normalized to Renilla. The mean relative luciferase activities (plus standard errors) obtained from three independent transfection experiments are shown.