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. 2006 Apr 6;25(8):1579–1589. doi: 10.1038/sj.emboj.7601051

Figure 1.

Figure 1

HOPS complex purification. (A) Lysates from BJ2168 (no tag) and CSY12 (Vps33-SBP) vacuoles were incubated with streptavidin Sepharose. Bound material was eluted in buffer containing Triton X-100 as described in Materials and methods and subjected to SDS–PAGE and silver staining. Lane 1, material purified from BJ2168 vacuoles; lane 2, material purified from CSY12 vacuoles. (B) Lysates from BJ3505 (lanes 1–3) and CSY12 (lanes 4–6) vacuoles were incubated with streptavidin Sepharose. Bound material was eluted in the presence of BSA and subjected to SDS–PAGE and immunoblotting. Lanes 1 and 4, vacuole lysate (representative of 10% of sample); lanes 2 and 5, material not bound to streptavidin Sepharose (representative of 10% of sample); lanes 3 and 6, material eluted from streptavidin Sepharose (representative of 100% of sample).