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. Author manuscript; available in PMC: 2007 Apr 1.
Published in final edited form as: Gastroenterology. 2006 Apr;130(4):1283–1300. doi: 10.1053/j.gastro.2006.01.010

Fig. 1. Adenovirus mediated increase in hepatic HNF6 expression following partial hepatectomy.

Fig. 1

Two-month-old CD-1 mice were subjected to tail vein injections of either adenovirus CMV-LacZ (AdLacZ) or adenovirus CMV-HNF6 (AdHNF6). Two days after injection, mice were subjected to partial hepatectomy (PHx) operation and remnant regenerating livers were harvested at indicated time points following PHx. At least three regenerating mouse livers were used for each time point. (A) AdHNF6 infected regenerating liver displayed increased levels of HNF6 mRNA. RNase protection assays were performed to detect mouse HNF6 expression in either AdLacZ infected or AdHNF6 infected livers at the indicated time points following PHx and Cyclophilin levels were used as a loading. (B-I) AdHNF6 infection increases nuclear levels of HNF6 protein in regenerating hepatocytes. We used affinity purified HNF6 antibody for immunohistochemical staining of liver sections at the indicated hours following PHx from mice infected with either AdLacZ (B, D, F and H) or AdHNF6 (C, E, G and I). Micrographs are at 200X magnification.