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. Author manuscript; available in PMC: 2007 Apr 1.
Published in final edited form as: Gastroenterology. 2006 Apr;130(4):1283–1300. doi: 10.1053/j.gastro.2006.01.010

Fig. 3. Increased expression of Cyclin D1, Forkhead Box m1, Tumor Growth Factor α and Cdk2 during liver regeneration in AdHNF6 infected mice.

Fig. 3

(A–B) Increased hepatic mRNA levels of Forkhead Box m1 (Foxm1) transcription factor, Cyclin D1 and Tumor Growth Factor α (TGFα) in regenerating livers from AdHNF6 infected mice. Total RNA was isolated from regenerating liver of mice that were infected with either AdLacZ or AdHNF6 and analyzed for mRNA expression by RNase protection assays (in triplicate) with probes specific to either HNF6 (B), TGFα (B), Foxm1 (A) or Cyclin D1 (A). Note that representative duplicate samples are shown and that Cyclophilin or L32 levels were used to normalize expression levels. Mean mRNA expression levels of 2-month old CD-1 mouse liver at either 0 hours (A) or 32 hours post PHx (B) were set at 1.0. (C) Increased protein levels of Cyclin D1 and Cdk2 during regenerating livers in AdHNF6 infected mice. Total protein extracts were isolated from regenerating liver and analyzed for Cdk2 and Cyclin D1 expression by Western blot analysis. Note that representative duplicate samples from two distinct regenerating mouse livers are shown and that β–Actin protein band was used to normalize expression levels. Quiescent mock-infected liver (MI) was set at 1.0. The asterisk indicates statistically significant changes: *P ≤ 0.05.