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. Author manuscript; available in PMC: 2007 Apr 1.
Published in final edited form as: Gastroenterology. 2006 Apr;130(4):1283–1300. doi: 10.1053/j.gastro.2006.01.010

Fig. 4. HNF6 and Foxm1 proteins activate transcription of the Cyclin D1 and Tumor Growth Factor α promoter regions in cotransfection assays.

Fig. 4

(A) AdHNF6 infection of HepG2 cells increases mRNA expression of endogenous human Tumor Growth Factor α (TGFα) gene. HepG2 cells were either mock infected (MI) or infected with AdLacZ (LacZ) or AdHNF6 (H6) and 24 hours after infection, RNA was isolated and analyzed for mRNA expression by RNase protection assays (RPA) with probes specific to either HNF6 or TGFα. (B–C) CMV-HNF6 and CMV-FoxM1b expression vectors activate transcription of the Cyclin D1 and Tumor Growth Factor α promoter regions in cotransfection assays. HepG2 cells were transfected with the −1.7 Kilobase pair (KB) mouse TGFα promoter (B) or the −1.7 KB human Cyclin D1 promoter (C) Luciferase plasmid and either CMV HNF-6 cDNA (HNF6) or CMV empty expression vectors. At 24 hours after transfection, the cells were used to prepare cell extracts, which were then analyzed for dual Luciferase enzyme activity as described in Materials and Methods. We also examined whether cotransfection of these promoters with the CMV human FoxM1b expression vector (FoxM1b) could stimulate expression of these promoter regions and synergize with the HNF6 transcription factor (HNF6 + FoxM1b). Results are presented as mean fold induction of promoter activity ± SD from two separate experiments in triplicate, with the CMV empty value set at 1.0. The asterisks indicate statistically significant changes: *P ≤ 0.05 and **P ≤ 0.01.