FIGURE 5.

NPH-II but not DED1 actively displaces U1A from the RNA. (A) Displacement of U1A by NPH-II (representative PAGE). Protein displacement is indicated by the ability of the DExH/D protein to separate the RNA strands with U1A bound (lane 3, Jankowsky et al. 2001). Mobilities of the RNA complex and the single-stranded RNA are indicated by cartoons on the left. Reactions were allowed to proceed for 5 min. Lanes are as follows: (1) NPH-II only; (2) NPH-II and ATP; (3) NPH-II and ATP with U1A; (4) boiled control. (B) Inability of DED1 to actively displace U1A from the RNA (representative PAGE). Lanes are as follows: (1) DED1 only; (2) DED1 and ATP; (3) DED1 and ATP with U1A; (4) boiled control.