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. 2006 May;12(5):765–774. doi: 10.1261/rna.2292106

FIGURE 8.

FIGURE 8.

Spliceosome recycling in U6-overproducing Δ25 extracts. (A) Wild-type (lane 1), Δ25 (lane 2), and U6-overproducing Δ25 (lane 3) extracts were subjected to electrophoresis on native polyacrylamide gels, and RNA was analyzed by Northern blotting. (U6−) Not overproducing U6, (U6+) overproducing U6. (B) Spliceosome recycling assays were carried out as in Figure 7 with onefold (0.4 nM), fivefold (2 nM), or 10-fold (4 nM) excessive amounts of unlabeled wild-type over labeled pre-mRNA in wild-type (lanes 1–4), Δ25 (lanes 6–10), or U6-overproducing Δ25 extracts (lanes 11–14) supplemented with affinity-purified NTC at a concentration of ∼5 nM. (WT) Wild type, (Δ25/U6−) Δ25 not overproducing U6, (Δ25/U6+) U6-overproducing Δ25.