FIGURE 1.

Inhibition of cap-dependent translation in HeLa cells by CBV infection. (A) Total RNA was isolated from HeLa cells infected with CBV from 0 to 8 h. Replication of CBV RNA was analyzed by Northern blot. (B) Radiolabeled extracts from HeLa cells infected with CBV from 0 to 12 h were separated by SDS-PAGE. Total protein synthesis was examined by autoradiograph. (C) Extracts from samples shown in B were separated by SDS-PAGE and immunoblotted with antibodies directed against eIF4G and PABP to detect full-length (solid arrows) and cleavage products (open arrowheads) of the respective proteins.