FIGURE 1.

RDE-4 protein constructs. (A) Schematic diagram of RDE-4 protein constructs with double-stranded RNA binding motifs (dsRBMs) shown as boxes and the molecular weights indicated. (B) Amino acid sequence of RDE-4. Vertical arrows indicate the last amino acid before the engineered stop codon of truncations 2 and 3. Horizontal arrow indicates start position for the C terminus protein. Truncation 2 contains eight additional nonnative amino acids (GMPPNNCS) at its C terminus (see Materials and Methods). (C) SYPRO Red-stained SDS-PAGE gel of purified RDE-4, Truncations 2 and 3 (Trc2 & Trc3) and C terminus (Cterm).