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. 2006 May;12(5):862–871. doi: 10.1261/rna.2319806

FIGURE 4.

FIGURE 4.

Decreased association of Snu114 with Prp8 and Brr2 in snu114–12 and snu114–40 extracts. (A) Western blot of TCA extracts showing the levels of Prp8 and Snu114 in the indicated strains. (B) Western blot of bead-beat extracts showing total levels of Prp8 and Snu114. (C) Snu114 was immunoprecipitated using polyclonal antibodies. A Western blot of the precipitated proteins was probed for Snu114 and Prp8. As a control for the IP, SNU114 extract was incubated with nonimmune serum (NIS) conjugated to Protein A Sepharose. (D) Levels of Prp8, Brr2-TAP, and Snu114 in TAP-tagged Brr2 strains in bead-beat extract. (E) Brr2-TAP was affinity-purified, and a Western blot of the precipitated proteins was probed for Prp8, Brr2-TAP, and Snu114. Note that Snu114–60 migrates faster than Snu114 because the protein contains 70 fewer amino acids. In A, B, and D, Rpl3 was used as a loading control.