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Reverse transcriptase analysis of the positions of the DMS and CMCT modifications in 16S rRNA. (A) Positions U1414 and A1418, (B) positions U1483 and A1495, (C) position U793, and (D) position A702, whose modification interferes with 30S reassociation, are indicated by arrows. 16S, 30S, and 70S denote naked 16S rRNA or 16S rRNA extracted from the free 30S subunits or from the 70S ribosomes, respectively. The sequencing lanes are indicated by A, C, G, and T.
