FIGURE 4.
Modeling of interfering positions into the crystal structure of T. thermophilus 30S subunits (Schluenzen et al. 2000; Wimberly et al. 2000) (PDB accession code 1FKA). (A) The left view is from the A-site side, and (B) the right view is from the subunit interface side. Arrows indicate interfering positions (E. coli numbering), and intersubunit bridges (B2a–B7a) are in the brackets. Interfering positions are highlighted in red and yellow spacefill. The rest of the rRNA is in blue wireframe, and r-proteins are in cyan spacefill (RasWin molecular graphics). Interfering position U793 is not resolved in the crystal structure of the T. thermophilus 30S subunit and therefore is not indicated in the figure. (C) Upper part of the 16S rRNA helix 44. Interfering positions (U1414, A1418, A1483, and U1495) are displayed in stick format. G1486 forms a base pair with U1414, which, in turn, is a part of bridge B3. Nucleotides, which form intersubunit bridges (B2a, B3, and B5), are indicated in different colors (B2a, green; B3, cyan; and B5, blue). The rest of the rRNA is in the black wireframe mode.