Table 2.
Comparison of FragLib to the Prior Library-based Work in Carbohydrate Sequencing
| approach/tool | glycan sample preparation | reference spectra generation method | MSn or MS/MS | reference structure entity | reference spectral library management |
|---|---|---|---|---|---|
| FragLib | permethylated with metal ion adduct | empirical | MSn | glycan substructure | computer-based |
| catalog library | native glycan | empirical | MSn | glycan substructure | manual |
| GlycoFragment/ GlycoSearchMS | native glycan | theoretical | MS/MS | intact glycan | computer-based |
| Glycosid IQ | native glycan | theoretical | MS/MS | intact glycan | computer-based |
| trisaccharide MS2 library | native glycan | empirical | MS/MS | glycan substructure | manual |