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(A) Zygote with an oil drop marking the position of the fertilization cone.
(B) The embryo after hypotonic treatment. The cytoplasmic protrusion marks the site of ZP penetration by the sperm. Arc distance (θ) between the point of ZP penetration and fertilization is measured.
(C) Distribution of the arc distance between the sites of ZP penetration and fertilization.
Scale bar in (A) represents 20 μm.
