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. Author manuscript; available in PMC: 2006 Apr 21.
Published in final edited form as: Nat Genet. 2005 Jul 17;37(8):873–877. doi: 10.1038/ng1606

Figure 3.

Figure 3

Co-segregation of closely spaced alleles in skeletal muscle of double heteroplasmic individuals argues against recurrence of mutations. In two-round PCR reactions, products specific for either one (A) or the other (G) allele of heteroplasmic nucleotide position 185 were amplified first, then proportions of the other heteroplasmic mutation were determined in both allele-specific reactions by mismatch-PCR and restriction digestion. a, Triplasmic and tetraplasmic distributions of A185G heteroplasmy with A189G heteroplasmy in skeletal muscle. Note the lack of the digested band in the ‘G’ lanes for most individuals, and its presence in individuals p13 and p15. b, Triplasmic segregation of A185G heteroplasmy with a heteroplasmic single nucleotide insertion at position 195. c, Triplasmic distribution of 185 and 189 alleles in individual muscle fibers of individual p3 (Figure 3a). Note the absence of data points in the upper right corner corresponding to the 185G/189G haplotype.