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. Author manuscript; available in PMC: 2006 Apr 21.
Published in final edited form as: Arch Biochem Biophys. 2006 Jan 1;445(1):115–123. doi: 10.1016/j.abb.2005.11.005

Table 1.

Purification of a recombinant hCE-1 and hCE-2 produced in insect cells

Step Total activitya (nmol/min) Total protein (mg) Activity (%) Specific activity (nmol/min/mg) Purification fold
hCE-1
Supernatant 10,000g 224 721 100 0.31 1
Supernatant 100,000g 213 607 95 0.35 1.1
DEAE 57 24 25 2.4 7.7
Preparative IEF 17 1.2 7.6 14 44
hCE-2
Supernatant 10,000g 218 588 100 0.37 1
Supernatant 100,000g 195 423 89 0.46 1.2
DEAE 110 35 50 3.2 8.6
Preparative IEF 20 1.1 9.2 18 49
a

Enzyme activity was measured using A3 as described under “Materials and methods.”