Table 1.
Step | Total activitya (nmol/min) | Total protein (mg) | Activity (%) | Specific activity (nmol/min/mg) | Purification fold |
---|---|---|---|---|---|
hCE-1 | |||||
Supernatant 10,000g | 224 | 721 | 100 | 0.31 | 1 |
Supernatant 100,000g | 213 | 607 | 95 | 0.35 | 1.1 |
DEAE | 57 | 24 | 25 | 2.4 | 7.7 |
Preparative IEF | 17 | 1.2 | 7.6 | 14 | 44 |
hCE-2 | |||||
Supernatant 10,000g | 218 | 588 | 100 | 0.37 | 1 |
Supernatant 100,000g | 195 | 423 | 89 | 0.46 | 1.2 |
DEAE | 110 | 35 | 50 | 3.2 | 8.6 |
Preparative IEF | 20 | 1.1 | 9.2 | 18 | 49 |
Enzyme activity was measured using A3 as described under “Materials and methods.”